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UVAS Short Training by IBBT on “Basic Molecular Biology Techniques

UVAS Short Training By IBBT On “Basic Molecular Biology Techniques

UVAS Short Training by IBBT on “Basic Molecular Biology Techniques

Introduction

Molecular biology deals with the molecular basis of biological activity. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of a cell, including the interactions between the different types of DNA, RNA and protein biosynthesis as well as learning how these interactions are regulated. The basic techniques of molecular Biology are DNA extraction from variety of source samples, Polymerase Chain Reaction (PCR), and Real Time PCR. DNA extraction is the removal of deoxyribonucleic acid (DNA) from viruses or other cells to perform a number of processes including the sequencing of genomes, detection of bacteria and viruses in the environment, detection of diseases, and many other forensic and diagnostic processes. The polymerase chain reaction (PCR) is a biochemical technology in molecular biology used to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. A quantitative polymerase chain reaction (qPCR), also called real-time polymerase chain reaction, is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR), which is used to amplify and simultaneously quantify a targeted DNA molecule. These techniques have widespread applications in many areas of genetic analysis such as Medical applications, Infectious disease applications, Forensic applications and research application.

Who should attend?

Any PCR users who have an interest in starting to use real-time PCR or current new users who want further training on Gene Expression Applications: Absolute and Relative Quantitation, Theory, Analysis and Optimization of PCRs.

Objectives

  • To better understand the basic techniques of Molecular Biology
  • To update the participants with latest applications of DNA and PCR
  • To demonstrate real-time PCR for understanding of its applications in academia and industry
  • To promote the usage of basic Molecular Biology techniques in life sciences research

Program

Tuesday 08th July, 2014

09:00 AM-09:15 AM: Introduction of the course by Prof. Dr. Tahir Yaqub
09:15 AM-10:15 AM: Lecture on DNA Extraction by Dr. Asif Nadeem /Dr. Wasim Shehzad
10:15 AM-1:00PM: Practical Training of DNA Extraction: From Blood Samples by Ms. Maryam Javed.
From Tissue Samples by Dr. Sehrish Firyal / Ms. Lala Rukh.

Wednesday 09th July, 2014

9:00 AM-10:00 AM: Lecture on Polymerase Chain Reaction (PCR) by Dr. Ali Raza Awan/Dr. M. Imran
10:00 AM-12:00 PM: Practical Training of Polymerase Chain Reaction (PCR) by Dr. M. Yasir Zahoor/Dr. M. Imran

Thursday 10th July, 2014

09:00 AM-10:00 AM: Lecture on Real Time PCR (RT-PCR) by Dr. M. Zubair Shabir/Dr. M. Wasim
10:00 AM-1:00 PM: Practical Training of Real Time PCR (RT-PCR) Session I by Dr. M. Zubair Shabir/Dr. M. Nauman Zahid
2:00 PM-3:30 PM: Practical Training of Real Time PCR (RT-PCR) Session II by Dr. M. Zubair Shabir /Mr. Rashid Saif.
3:30 PM 4:00 PM: Concluding Session and Certificate Distribution Ceremony

Goals

At the end of the course the attendee will be familiar with the theory and practice of PCR and real time PCR and one will learn the most cost-effective way to set-up, run and analyze the basic molecular biology experiments.

Contents

  • DNA Extraction
  • Polymerase Chain Reaction
  • Real Time PCR
  • See Detailed Contents

Fees

  • Rs. 4,000 for faculty/entrepreneurs
  • Rs 2,000 for students
  • Lunch and tea will also be served

 

Brochure

Course Programme

Registration Form

Laste Date of Registration : 30th June 2014

Venue

Institute of Biochemistry and Biotechnology
(IBBT)
University of Veterinary and Animal
Sciences, Lahore

Contact

Prof. Dr. Tahir Yaqub
Director
Institute of Biochemistry & Biotechnology
University of Veterinary and Animal Sciences
Lahore-Pakistan
0300-6950418
042-99210256

Dr. Ali Raza Awan
Assistant Professor
(0321-8442090)
arawan77@uvas.edu.pk

Dr. M. Yasir Zahoor
Assistant Professor
yasir.zahoor@uvas.edu.pk

UVAS Short Training By IBBT On “Basic Molecular Biology Techniques

International Max Planck Research School, Ph.d Fellowship for Molecular and Cellular Life Sciences,

International Max Planck Research School, Ph.d Fellowship for Molecular and Cellular Life Sciences

Requirements
 

Please first clarify which program options you intend to apply for and check the eligibility criteria.

Program options
a.) Regular PhD program
Entry level for doctoral students is usually a Master of Science degree or an equivalent degree. This could be either a German Diploma, MSc or other related degrees. A final decision regarding eligibility can only be made after submission of a complete application and requires the approval of university officials. In case of questions regarding eligibility it is recommended to get in touch with the IMPRS coordination office.

b.) Fast track program
Please note that the fast track program is currently not available

Application form 
The application form contains eight different tabs and allows you to enter all required information and to upload corresponding documents. You may revisit and modify your personal application form any time prior to submission. Once your application is complete, please submit it by clicking the “submit” button in the upper menu. After submission you will no longer be able to edit the entries (except name, password and email). However, you will be able to view your submitted data throughout the application process.
Submission of applications is only possible, if all required fields (marked with a yellow triangle) are filled out. Non required fields are optional but it is recommended to fill out all those fields that apply to your specific application. The more information you provide the better we will be able to assess your application.
Once you have submitted your application, the coordination office will check whether your application is complete (i.e. check whether you have uploaded all required documents).  The status of your application is summarized in the first tab of the application form.

Language skills
Applicants need to be fluent in written and spoken English and, unless native speakers, should document their proficiency in English. Ideally, this can be achived with a TOEFL report (institutional code of IMPRS-LS: 7426), an IELTS report or a Cambridge ESOL certificate (for minimal language requirements see here). German language skills are not required but interested international students will be offered opportunities to take German language courses.

Documents for PhD program: 
Required documents are:

  • Curriculum Vitae (CV)
  • Transcript (record of study) and degree certificate of bachelor degree, Vordiplom or equivalent degree
  • Transcript (record of study) and degree certificate of master degree, Diplom or equivalent degree (if applicable). If your degree program is not yet completed, please provide temporary transcripts
  • Two letters of recommendation (more details see below)

Recommended documents are:

  • certificate of proficiency in English (recommended if not native English speaker or education was not in English)
  • High school degree transcripts or equivalent
  • scholarships, prizes and awards (if applicable)
  • copy of passport (international applicants only)
  • portrait picture
  • A GRE subject test in either biochemistry, biology, chemistry or physics (Institutional code is: 2828 Intl Max Planck Res Sch Life Sci) A GRE subject test is recommended if you come from a non EU country but will strongly support any application; for applicants from India we also accept GATE)

Letters of recommendation:

Please get in touch with your referees as soon as possible and get their affirmation to write a recommendation on your behalf. Then fill out the tabs for referee contacts in the application form and follow further instructions. Once authorized by you, the coordination office will invite the referee to submit his/her letter of recommendation electronically.

As soon as a letter of recommendation on your behalf has been submitted, we will send your referee a confirmatory email. The submission of a reference on your behalf is also indicated in the status box of your application form.
Please keep in mind that your application can only be processed after we have received the letters of recommendation. Applications that lack these letters after the deadline for submission has expired will be considered incomplete and will therefore not be processed any further. It is your responsibility, to make sure that the referees submitl the letters of recommendation to the coordination office in a timely manner.

Translation of documents:
All submitted official documents must be in English or in German or need to be translated into either one of these languages. Please submit a copy of the original document and the translation.
For the online application non-certified translations are sufficient.  However, please keep in mind that originals (or certified copies thereof) and certified translations of documents submitted in the application process will have to be presented to the coordination office for verification purposes, once candidates have been accepted to the program. Certification of copies can be issued, for example, by the dean’s or registrar’s office of your university, a German embassy or consulate, a branch of the German Academic Exchange Service (DAAD), a public notary or a Goethe Institut.

Accepted file formats:
Please only submit PDF files or JPEG files. Other document types will not be processed and consequently will not be visible to the reviewers.If a document consists of more than one page it is strongly recommended to combine those pages into a single PDF file. This can be done easily by using Acrobat or other PDF generating programs that are available as freeware.Please do not submit (password) protected documents. Protected documents cannot be converted into a final PDF file and thus will not be seen by the reviewing professors. This may adversely affect your evaluation.For file names, please only use standard characters from the latin alphabet and numbers. Please avoid special characters such as inverted commas, plus sign or question marks just to mention a few. These characters may be incompatible with UNIX and hence we will be unable to open your file. It is recommended to use file names that reflect the content of the file. All uploads have a size restriction of 4 MB each, so please take care that uploads are not too big. We recommend scanning documents with no more than 300dpi. It is recommended to open uploaded files after upload in order to confirm integrity of the file.

Final PDF document
For each submitted application, a final PDF file will be generated. Documents submitted will be arranged in the following order (this is how the professors will see your application):

  • cover page with name of applicant, registration number, portrait picture, scientific interests, essays and additional information about the candidate
  • CV
  • letters of recommendation
  • transcripts and degree certificates
  • honors, scholarships, prizes and awards (including the respective explatation provided by applicant)
  • English language proficiency information and certificates
  • all other documents submitted
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